PXD076272 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Novel Approach for Generating Antigen Interference-Free Anti-Peptide Polyclonal Antibodies for Quantitative Measurements of Low-Abundance Protein Biomarkers Using Ultra-Sensitive Anti-Peptide Immunocapture LC-MS/MS Assays |
| Description | Antigen interference from residual antigen peptides remains a persistent challenge in anti-peptide immunocapture liquid chromatography-tandem mass spectrometry (IC-LC-MS/MS) assays for quantifying low-abundance protein biomarkers. In this manuscript, we introduce a novel approach that utilizes carrier protein-conjugated, stable isotopically labeled surrogate peptides as antigens to generate anti-peptide polyclonal antibodies (pAbs). By leveraging mass spectrometry to distinguish residual labeled antigen peptides from endogenous peptides, this method effectively eliminates antigen interference in anti-peptide IC-LC-MS/MS assays. This innovative approach significantly advances anti-peptide IC-LC-MS/MS quantitative bioanalysis for targeted proteomics, enabling the detection of low-abundance protein biomarkers at picomolar and sub-picomolar levels. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-04 |
| AnnouncementXML | Submission_2026-09-04_14:11:43.318.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Huidong Gu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q TRAP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-27 14:30:52 | ID requested | |
| ⏵ 1 | 2026-09-04 14:11:43 | announced | |
Publication List
Keyword List
| submitter keyword: Stable isotopically labeled peptide antigen, Anti-peptide immunocapture, Protein biomarker, Antigen interference, LC-MS/MS |
Contact List
| Dr. Jim Shen |
|---|
| contact affiliation | Bioanalysis and Translational Mass Spectrometry, Bristol Myers Squibb |
| contact email | Jim.Shen@bms.com |
| lab head | |
| Huidong Gu |
|---|
| contact affiliation | Bristol Myers Squibb |
| contact email | huidong.gu@bms.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD076272 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076272
- Label: PRIDE project
- Name: A Novel Approach for Generating Antigen Interference-Free Anti-Peptide Polyclonal Antibodies for Quantitative Measurements of Low-Abundance Protein Biomarkers Using Ultra-Sensitive Anti-Peptide Immunocapture LC-MS/MS Assays
