PXD076253

PXD076253 is an original dataset announced via ProteomeXchange.

Title	Basil essential oil modulates the stress response of Klebsiella variicola: A label-free proteomic study
Description	Klebsiella variicola, traditionally considered a plant-associated species, is now recognized as an emerging foodborne and opportunistic pathogen at the human–animal–environment interface. Its ecological versatility, ability to colonize plants and food matrices, and increasing reports of antimicrobial resistance make this bacterium a growing concern for both public health and food safety. As the demand for alternative and clean-label antimicrobial strategies intensifies, essential oils (EOs) have gained attention for their broad biological activities and multi-target mechanisms. Among these, basil (Ocimum basilicum) essential oil (BEO), rich in linalool and phenylpropanoids, exhibits antimicrobial activity primarily through membrane disruption, metabolic interference, and oxidative imbalance. Despite the expanding use of plant-derived antimicrobials, the cellular response of K. variicola to EOs remains largely unexplored. To address this gap, this study employed a label-free quantitative proteomic approach to characterize the global response of K. variicola to a subinhibitory concentration of BEO. Exposure to BEO markedly affected proteins involved in membrane homeostasis, oxidative stress management, and translation processes. The concurrent repression of energy-related pathways and activation of antioxidant defences indicates that BEO imposes multifactorial physiological stress rather than acting through a single inhibitory target. These findings provide the first proteome-level insight into EO action against K. variicola and highlight molecular vulnerabilities that may reduce its persistence in food-processing environments. Overall, this study supports BEO as a promising natural antimicrobial and offers novel mechanistic clues on how emerging Klebsiella species respond to plant-derived stressors.
HostingRepository	PRIDE
AnnounceDate	2026-08-06
AnnouncementXML	Submission_2026-08-06_04:06:23.026.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD076253
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Valentina Pavone
SpeciesList	scientific name: Klebsiella sp. 'Medkleb'; NCBI TaxID: NEWT:2729543;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 09:21:27	ID requested
⏵ 1	2026-08-06 04:06:23	announced

10.1093/JAMBIO/LXAG152;

10.6019/PXD076253;

submitter keyword: Klebsiella variicola

Ocimum basilicum

natural preservatives

label-free proteomics

membrane disruption

antimicrobial activity

Mirella Luciani
contact affiliation	IZSAM
contact email	m.luciani@izs.it
lab head
Valentina Pavone
contact affiliation	IZSAM
contact email	v.pavone@izs.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076253
     1. Label: PRIDE project
     2. Name: Basil essential oil modulates the stress response of Klebsiella variicola: A label-free proteomic study