PXD076239 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | F-actin dynamics couples sphingolipid metabolism to epithelial barrier integrity in chronic colitis (proteomics) |
| Description | Intestinal barrier dysfunction is a hallmark of gastrointestinal disorders, including inflammatory bowel diseases (IBD). In IBD, the disruption of the gut barrier causes liquid loss and persistent immune response. Understanding the cellular events underlying epithelial barrier disruption during chronic inflammation is essential for targeting increased intestinal permeability. Filamentous actin (F-actin) destabilization accompanied by metabolic dysfunction has been previously reported in the Muc2 knockout colitis model; however, the mechanistic contribution of these defects to chronic intestinal barrier loss remains unclear. Here, we identify impaired cytoskeleton dynamics as a critical driver of intestinal barrier dysfunction in chronic colitis. An imbalance between polymeric and monomeric actin disrupts the epithelial barrier in both 3D organoid system and in vivo. Actin and associated factors were identified as primary interactors of claudin-3, and this interaction was reduced under chronic inflammatory conditions in vivo. Further analysis revealed ceramide metabolism as a potential metabolic regulator of actin dynamics and barrier integrity during chronic inflammation. Consistently, intestinal samples from IBD patients showed concurrent disruption of tight and adherens junctions and reduced F-actin levels. Together, these findings reveal F-actin dynamics as one of the key mechanisms of barrier dysfunction in IBD and highlight ceramide metabolism as a potential therapeutic target in IBD. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-01 |
| AnnouncementXML | Submission_2026-04-01_00:05:29.702.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Snezhanna Medvedeva |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-27 03:58:55 | ID requested | |
| ⏵ 1 | 2026-04-01 00:05:30 | announced | |
Publication List
Keyword List
| submitter keyword: Cldn3, IBD, actin |
Contact List
| Elena Kozhevnikova |
|---|
| contact affiliation | Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences |
| contact email | kozhevnikova@mcb.nsc.ru |
| lab head | |
| Snezhanna Medvedeva |
|---|
| contact affiliation | Institute of Molecular and Cellular Biology SB RAS |
| contact email | medvedeva@mcb.nsc.ru |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076239
- Label: PRIDE project
- Name: F-actin dynamics couples sphingolipid metabolism to epithelial barrier integrity in chronic colitis (proteomics)
