PXD076233

PXD076233 is an original dataset announced via ProteomeXchange.

Title	The composition of periostracum layer in the razor clam Sinonovacula constricta and its response to sulfide
Description	The razor clam Sinonovacula constricta inhabits sulfide-rich intertidal sediments and exhibits remarkable tolerance to this toxicant, yet the role of its periostracum layer in sulfide adaptation remains poorly understood. In this study, we investigated the composition, structure, and sulfur-responsive expression of periostracum proteins. Scanning electron microscopy and energy-dispersive X-ray spectroscopy revealed that the periostracum layer is approximately 10 μm thick and contains 1.43 wt% sulfur, suggesting its involvement in sulfur deposition. Using LC-MS/MS, we identified 77 high-confidence proteins from the periostracum, which were classified into six functional categories: enzymes, framework proteins, immune-related proteins, calcium ion-related proteins, other proteins, and proteins with unknown functions. Phylogenetic analyses of representative proteins revealed bivalve-specific evolutionary patterns, with several proteins exclusively present in Bivalvia. Quantitative PCR analysis under sulfide exposure (10 μM and 100 μM Na₂S) showed that genes encoding chitin-binding protein 3 and tyrosinase were significantly upregulated in the mantle, whereas collagen and cadherin 3 were generally suppressed. These results indicate that the periostracum layer is not merely a passive physical barrier but a dynamic biological interface that responds to sulfide stress through coordinated regulation of structural and enzymatic proteins. This study provides the first comprehensive proteomic profile of the razor clam periostracum and highlights its potential role in sulfide tolerance, offering insights for sulfur-tolerant aquaculture breeding and bioremediation applications.
HostingRepository	iProX
AnnounceDate	2026-03-27
AnnouncementXML	Submission_2026-03-27_02:05:49.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Long
SpeciesList	scientific name: Sinonovacula constricta; NCBI TaxID: 98310;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 01:38:52	ID requested
⏵ 1	2026-03-27 02:05:50	announced

Dataset with its publication pending

submitter keyword: razor clam,sulfide,periostracum,genes

Chuang Liu
contact affiliation	Hohai University
contact email	chuangliu2020@hhu.edu.cn
lab head
Yu Long
contact affiliation	Hohai University
contact email	2929921415@qq.com
dataset submitter

iProX dataset URI