PXD076226

PXD076226 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation-mass spectrometry (IP-MS) of USP15-interacting proteins in 786-O cells
Description	786-O cells were lysed in IP buffer and processed for immunoprecipitation with anti-USP15 or normal rabbit IgG for 6 h at 4 °C. The immune complexes were incubated with Pierce Protein A/G Magnetic Beads (Thermo Scientific) overnight at 4 °C and washed with IP buffer. After SDS-PAGE and Coomassie brilliant blue staining, the gel was cut into sections and digested with trypsin. For each sample, 2 µg of total peptides were separated and analyzed with a nano-UPLC (nanoElute2) coupled to a timsTOF Pro2 mass spectrometer (Bruker) with a nano-electrospray ion source. Then the raw MS files were processed using PaSER software (Version 2023) and the built-in DDA-ProluCID-GPU search engine.
HostingRepository	iProX
AnnounceDate	2026-03-26
AnnouncementXML	Submission_2026-03-27_01:18:11.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yujie Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 01:17:43	ID requested
⏵ 1	2026-03-27 01:18:11	announced

Dataset with its publication pending

submitter keyword: USP15, IP-MS, 786-O

Zhiyuan Chen
contact affiliation	Renmin Hospital of Wuhan University
contact email	drchenzy@whu.edu.cn
lab head
Yujie Chen
contact affiliation	Renmin Hospital of Wuhan University
contact email	2020305232036@whu.edu.cn
dataset submitter

iProX dataset URI