PXD076118

PXD076118 is an original dataset announced via ProteomeXchange.

Title	Development of an in vitro model of Mycoplasma bovis infection in static bioreactors for proteomic investigation of small extracellular vesicles for biomarker discovery
Description	Mycoplasma bovis (M. bovis) is a pathogenic bacterium that causes significant production and welfare issues in cattle. The dairy industry is essential to New Zealand, contributing over a quarter of the country’s total exports. Each year, ~4.7 million cows produce ~20.5 billion litres of milk, which is worth ~$23 billion NZD to the New Zealand economy. An unregulated M. bovis outbreak would be catastrophic for the country. Eradication is challenging as M. bovis can infect host cells intracellularly, reducing detection from immunomodulatory cells. With an aim to improve diagnostics for post-eradication surveillance, small extracellular vesicles (sEVs) were used to identify novel biomarkers of M. bovis infection. Readily accessible in body fluids, these nanoparticles encapsulate protein cargo indicative of the metabolic state of their tissue of origin. Here, we developed a three-dimensional in vitro cell culture model using static bioreactors to investigate changes in host cell sEV protein cargo during M. bovis infection. Size exclusion chromatography columns were used to isolate sEVs from control and co-culture bioreactor flasks. Liquid chromatography-trapped ion mobility spectrometry-tandem mass spectrometry (LC–TIMS-MS/MS) was used to investigate the proteome of sEVs.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-05-31_16:09:06.088.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD076118
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Evelyne Maes
SpeciesList	scientific name: Metamycoplasma; NCBI TaxID: NEWT:2895509; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	carbamoylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-24 21:37:01	ID requested
⏵ 1	2026-05-31 16:09:07	announced

Pratt JT, Lake AVR, Maes E, Thomas A, Hefer C, Mitchell MD, Heiser A, Ross MA, infection alters small extracellular vesicle cargo derived from bovine endometrial epithelial cells cultured in static bioreactors. Front Microbiol, 17():1770401(2026) [pubmed]

10.3389/fmicb.2026.1770401;

10.6019/PXD076118;

submitter keyword: proteomics, small extracellular vesicles, bioreactor,Mycoplasma bovis

Evelyne Maes
contact affiliation	Bioeconomy Science Institute, AgResearch Ltd., Lincoln 7674, New Zealand.
contact email	evelyne.maes@agresearch.co.nz
lab head
Evelyne Maes
contact affiliation	Agresearch
contact email	evelyne.maes@agresearch.co.nz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076118
     1. Label: PRIDE project
     2. Name: Development of an in vitro model of Mycoplasma bovis infection in static bioreactors for proteomic investigation of small extracellular vesicles for biomarker discovery