PXD076099

PXD076099 is an original dataset announced via ProteomeXchange.

Title	Mouse Lymph node high endothelial venules
Description	High endothelial venules (HEVs) play a crucial role in adaptive immune responses in secondary lymphoid organs (SLOs). They are uniquely equipped with high levels of peripheral node addressins (PNAd), harboring carbohydrate structures that serve as L-Selectin ligands to efficiently facilitate lymphocyte homing. During inflammation, the HEV network expands in SLOs, increasing lymphocyte infiltration, but the underlying mechanisms maintaining HEVs remain underexplored. Here, we report that autophagy is essential for HEV function and expansion. Using mass spectrometry, and an inducible HEV tracer system in mice, we demonstrate that autophagy deficiency compromises LTR-signaling and the unfolded protein response in HEVs, leading to disrupted PNAd production, dedifferentiation, and reduced lymphocyte homing.Our work uncovers an unprecedented role of autophagy in safeguarding HEV identity and function during inflammation.
HostingRepository	PRIDE
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-07-10_04:22:22.721.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kathryn jacobs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	quadrupole orbitrap astral instrument

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-24 14:19:54	ID requested
⏵ 1	2026-07-10 04:22:23	announced

10.1016/J.IMMUNI.2026.06.020;

submitter keyword: inflammation, lymph node,HEVs

Gabriele Bergers
contact affiliation	VIB center for cancer biology, KU Leuven
contact email	gabriele.bergers@kuleuven.be
lab head
kathryn jacobs
contact affiliation	VIB center for cancer biology
contact email	katie7192@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD076099

PRIDE project URI

• PRIDE
  1. PXD076099
     1. Label: PRIDE project
     2. Name: Mouse Lymph node high endothelial venules