PXD076088

PXD076088 is an original dataset announced via ProteomeXchange.

Title	MiaA-mediated tRNA modifications couple tryptophan biosynthesis operon attenuation and changes in tRNA abundance to complex phenotypes in Pseudomonas aeruginosa
Description	tRNA-modifying enzymes are emerging as key regulators of bacterial physiology. MiaA, a tRNA isopentenyltransferase, is well studied in model organisms, but its role in the pathogen Pseudomonas aeruginosa remains unclear. Using LC–MS, nanopore sequencing, and transcriptional, translational and proteomic profiling, we mapped MiaA-dependent tRNA modifications and revealed unexpected effects of MiaA loss. Impaired translation of MiaA-sensitive codons reduced quorum-sensing–controlled virulence gene expression and attenuated pathogenicity in Galleria mellonella. Ribosome stalling at trp codons in miaA mutants caused overproduction of tryptophan- normally restrained by attenuation- along with upregulation of cognate tRNAs, linking translation to global metabolic adaptation. MiaA is tightly regulated and so central to bacterial physiology that its expression level correlates directly to virulence in clinical isolates, highlighting its role as a hub connecting translation, transcription, metabolism, and pathogenicity. These findings position MiaA as a key integrator of cellular processes critical for pathogen fitness and host interactions.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:11.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benedikt Kennepohl
SpeciesList	scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: NEWT:652611;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-24 11:23:53	ID requested
⏵ 1	2026-09-07 04:21:12	announced

Frommeyer YN, Th, ö, ming JG, Gomez NO, Grobe S, Preusse M, Arce-Rodr, í, guez A, Neubauer K, Kennepohl B, Kirk T, Franke R, Br, ö, nstrup M, Neumann-Schaal M, M, ü, sken M, Depledge DP, B, ä, hre H, Pich A, H, ä, ussler S, MiaA-mediated tRNA modifications couple tryptophan attenuation and changes in tRNA abundance to complex phenotypes in Pseudomonas aeruginosa. Nucleic Acids Res, 54(14):(2026) [pubmed]

10.1093/nar/gkag755;

submitter keyword: tRNA-modifying enzymes,Pseudomonas aeruginosa

Susanne Häußler
contact affiliation	Helmholtz Centre for Infection Research, Institute Molecular Bacteriology
contact email	susanne.haeussler@helmholtz-hzi.de
lab head
Benedikt Kennepohl
contact affiliation	Twincore Hannover
contact email	benedikt.kennepohl@helmholtz-hzi.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076088
     1. Label: PRIDE project
     2. Name: MiaA-mediated tRNA modifications couple tryptophan biosynthesis operon attenuation and changes in tRNA abundance to complex phenotypes in Pseudomonas aeruginosa