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PXD076049

PXD076049 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe cleavage sites of GmPSK4a were identified in vivo via IP-MS/MS
DescriptionThis project aims to determine the in vivo cleavage sites of GmPSK4a mediated by GmSBT1.2s via IP-MS/MS. GmSBT1.2b-HA and GmPSK4a-GFP were co-expressed in N. benthamiana and the total protein extract was incubated with GFP affinity beads for 3 hours at 4°C. The purified proteins were then separated by SDS-PAGE, and the specific cleaved products were excised and analyzed by LC-MS/MS to identify the cleavage site.
HostingRepositoryiProX
AnnounceDate2026-03-23
AnnouncementXMLSubmission_2026-05-26_00:29:47.268.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJingjing Lu
SpeciesList scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-03-23 20:33:31ID requested
12026-05-26 00:29:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cleavage sites, GmPSK4a, in vivo
Contact List
Xia Li
contact affiliationHuazhong Agricultural University
contact emailxli@mail.hzau.edu.cn
lab head
Jingjing Lu
contact affiliationHuazhong Agricultural University
contact email2445176049@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI