PXD076043

PXD076043 is an original dataset announced via ProteomeXchange.

Title	Cathelicidin derivative IDR-1018 exhibits anti-protease effects that alleviate Citrobacter rodentium-induced colitis
Description	Short, cationic, and amphipathic host defence peptides exhibit a wide range of anti-infective, anti-inflammatory, wound-healing, and antibiofilm activities. A synthetic peptide innate defence regulator (IDR) 1018, derived from the bovine neutrophil bactenecin, has been proposed as a therapeutic candidate for treating bacterial infections due to its immunomodulatory and antibacterial properties. IDR-1018 was examined as a treatment in murine colitis caused by the attaching/effacing enteropathogen, Citrobacter rodentium. IDR-1018, administered intra-peritoneally for three consecutive days after oral challenge with C. rodentium, mitigated microscopic lesions of colitis. Specifically, IDR-1018 reduced fecal protease levels, particularly elastase, by increasing the formation of inhibitory elastase–serpin complexes. IDR-1018 targeted neutrophils, the main effectors in colitis. Human and murine neutrophils stimulated and recruited by IDR-1018 exhibited reduced protease and elastase activity, as well as lower inflammatory cytokine production in response to inflammatory stimulation, showing functional reprogramming toward a less tissue-damaging phenotype. This study reveals a new activity of IDR-1018 in counteracting protease, which correspondingly attenuates C. rodentium colitis.
HostingRepository	PRIDE
AnnounceDate	2026-04-14
AnnouncementXML	Submission_2026-04-14_11:50:31.778.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Young
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-23 19:14:24	ID requested
⏵ 1	2026-04-14 11:50:32	announced

Dataset with its publication pending

submitter keyword: host defence peptides

colitis

Citrobacter rodentium.

Antoine Dufour
contact affiliation	Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, T2N 4N1, Canada.
contact email	Antoine.dufour@ucalgary.ca
lab head
Daniel Young
contact affiliation	University of Calgary
contact email	daniel.young1@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD076043

PRIDE project URI

• PRIDE
  1. PXD076043
     1. Label: PRIDE project
     2. Name: Cathelicidin derivative IDR-1018 exhibits anti-protease effects that alleviate Citrobacter rodentium-induced colitis