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PXD076004
PXD076004 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Integrated transcriptomic and proteomic analysis reveals the molecular mechanisms of Spodoptera litura response to Beauveria bassiana stress |
| Description | Spodoptera litura is a globally devastating agricultural pest that has developed severe resistance to chemical insecticides, necessitating environmentally sustainable biocontrol alternatives such as the entomopathogenic fungus Beauveria bassiana. However, the molecular mechanisms governing host-pathogen interactions in this system remain poorly understood. Here, we employed an integrated transcriptomic and proteomic approach to elucidate the molecular responses of S. litura larvae to B. bassiana infection across three spore concentrations (3.0×10⁴, 10⁶, and 10⁸ conidia/mL). Our multi-omics analysis revealed concentration-dependent transcriptional reprogramming, with moderate fungal load (10⁶ conidia/mL) eliciting the most extensive differential gene expression (2,434 DEGs), while proteomic responses remained remarkably consistent across treatments (437 DEPs per group). Functional enrichment analyses identified key defense modules including cuticle remodeling (chitin metabolism, cuticle proteins), detoxification pathways (glutathione S-transferases, cytochrome P450s), ion homeostasis, and protein quality control systems. Notably, we observed pervasive transcript-protein discordance, particularly at moderate concentration where nine immune-relevant genes exhibited strong transcriptional activation but corresponding proteins were predominantly downregulated, indicating dominant post-transcriptional regulation. Hub protein analysis revealed central regulatory nodes (A0A9J7EJ43, A0A9J7EMV7, A0A9J7IJC7) that likely coordinate multi-pathway immune responses. RNAi-mediated silencing of four candidate genes (SlPCP-C1B, SlCht10, SlGST1-1, and SlGST2) significantly enhanced fungal lethality, validating their protective roles. This study provides a comprehensive molecular framework for insect-fungal interactions, highlighting post-transcriptional regulation as a critical layer of immune control and identifying novel molecular targets for enhancing mycoinsecticide efficacy against this destructive pest. |
| HostingRepository | iProX |
| AnnounceDate | 2026-03-21 |
| AnnouncementXML | Submission_2026-03-23_01:15:58.909.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiayun Yao |
| SpeciesList | scientific name: Spodoptera litura; NCBI TaxID: 69820; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-23 01:14:14 | ID requested | |
| ⏵ 1 | 2026-03-23 01:15:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Spodoptera litura, Beauveria bassiana, proteomics, insect immunity, biocontrol |
Contact List
| Jianyu Meng | |
|---|---|
| contact affiliation | Guizhou Tobacco Science Research Institute, Guizhou University |
| contact email | mengjy0417@163.com |
| lab head | |
| Jiayun Yao | |
| contact affiliation | Guizhou Tobacco Science Research Institute, Guizhou University |
| contact email | jyyao1259@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
