PXD075983

PXD075983 is an original dataset announced via ProteomeXchange.

Title	UCHL3 regulates subgenomic flaviviral RNA condensates to promote virus propagation
Description	Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organised and maintained in cells remains poorly understood. Here we identify ubiquitin C-terminal hydrolase L3 (UCHL3) as a post-translational regulator of flavivirus sfRNA stability and function. Using a proximity-biotinylation sfRNA-interactome capture strategy (sfRAPID), we systematically identified sfRNA-interacting proteins during Zika virus infection. The engineered sfRNA construct contains flanking BoxB stem-loop structures that recruit a BirA-λN fusion protein (BASU), enabling biotinylation of proteins in close proximity to sfRNA and their subsequent isolation using streptavidin-conjugated beads. LC-MS/MS analysis of streptavidin pulldowns identified RNA-granule scaffolds and enzymes significantly enriched in sfRNA complexes relative to control RNA, including G3BP1, DDX10, DDX3, UBAP2L, OTUD4, RNase L, and UCHL3. This dataset provides an unbiased catalogue of the sfRNA-associated proteome during flavivirus infection, revealing that UCHL3 is incorporated into sfRNA-containing ribonucleoprotein assemblies where it functions as a proviral factor by suppressing RNase L activation and maintaining sfRNA within protective P-body compartments.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:20:57.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sumana Sanyal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-22 06:08:39	ID requested
⏵ 1	2026-09-07 04:20:58	announced

10.1002/advs.202521781;

Trejo-Cerro O, Beekmayer-Dhillon A, Teo QW, Siu L, Li MY, Heesom KJ, Pinto-Fern, á, ndez A, Sanyal S, UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation. Adv Sci (Weinh), 13(48):e21781(2026) [pubmed]

submitter keyword: dengue virus, flavivirus, biomolecular condensates,UCHL3, sfRNA, Zika virus, subgenomic RNA

Sumana Sanyal
contact affiliation	Sir William Dunn School of Pathology, University of Oxford
contact email	sumana.sanyal@path.ox.ac.uk
lab head
Sumana Sanyal
contact affiliation	University of Oxford
contact email	sumana.sanyal@path.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075983
     1. Label: PRIDE project
     2. Name: UCHL3 regulates subgenomic flaviviral RNA condensates to promote virus propagation