PXD075905

PXD075905 is an original dataset announced via ProteomeXchange.

Title	Identification of Lysine-Cysteine Crosslinking Sites in Homobifunctional NHS-Ester Crosslinkers
Description	NHS esters are commonly used reactive groups in crosslinking chemistry. We first demonstrated that NHS esters can react with the side-chain thiol group of cysteine and observed lysine-cysteine (K-C) crosslinks when using homobifunctional crosslinkers. However, in conventional crosslinking workflows, K-C crosslinks are difficult to identify. To address this, we explored factors affecting the stability of K-C crosslinks within the experimental pipeline. By employing an optimized workflow, we were able to consistently identify a greater number of K-C crosslinking sites while maintaining the detection of lysine-lysine (K-K) crosslinks.
HostingRepository	iProX
AnnounceDate	2026-03-19
AnnouncementXML	Submission_2026-03-19_20:43:12.299.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinfeng Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-19 20:42:01	ID requested
⏵ 1	2026-03-19 20:43:12	announced

Dataset with its publication pending

submitter keyword: XL-MS, DSS, DSSO, DSBU, GMBS, K-C cross-linking

Xiaoming Zhang
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	xmzhang@siii.cas.cn
lab head
Xinfeng Yang
contact affiliation	Suzhou Medical College of Soochow University
contact email	yxf13583448015@163.com
dataset submitter

iProX dataset URI