PXD075878 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The translatome of quiescent Plasmodium falciparum gametocytes reveals parasite pyridoxal 5’-phosphate (PLP) biosynthesis is essential for efficient mosquito stage development |
| Description | The ability of Plasmodium falciparum gametocytes to remain quiescent within the vertebrate host but poised for rapid onward development in the mosquito is an adaptation essential to maximise the onward spread of malaria. In this dormant state, mature infectious stage V gametocytes are largely unaffected by most antimalarial drugs and our limited understanding of how gametocytes prepare for mosquito transmission has hindered the identification of new molecular targets for transmission-blocking therapeutics. In this study, we move beyond the total proteome of gametocytes and define the translatome of mature stage V gametocytes using L-azidohomoalanine incorporation into nascent proteins, click chemistry purification and proteomic analysis. We identify the proteins and pathways that gametocytes sustain in preparation for transmission during this dormant period and through genetic disruption, we validate this approach by demonstrating the importance of parasite pyridoxal 5’-phosphate biosynthesis for mosquito transmission. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-25 |
| AnnouncementXML | Submission_2026-03-25_09:28:14.376.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jack Houghton |
| SpeciesList | scientific name: Plasmodium falciparum; NCBI TaxID: NEWT:5833; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-19 13:35:12 | ID requested | |
| ⏵ 1 | 2026-03-25 09:28:14 | announced | |
Publication List
Keyword List
| submitter keyword: malaria, Plasmodium falciparum, translatome |
Contact List
| Michael Delves |
|---|
| contact affiliation | London School of Hygine and Tropical Medicine |
| contact email | michael.delves@lshtm.ac.uk |
| lab head | |
| Jack Houghton |
|---|
| contact affiliation | Imperial College London |
| contact email | j.houghton@imperial.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD075878 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075878
- Label: PRIDE project
- Name: The translatome of quiescent Plasmodium falciparum gametocytes reveals parasite pyridoxal 5’-phosphate (PLP) biosynthesis is essential for efficient mosquito stage development
