PXD075836

PXD075836 is an original dataset announced via ProteomeXchange.

Title	Mammalian MemPrep establishes the lipid composition ER membranes in HEK293T cells
Description	Mammalian MemPrep is an approach that enables the isolation of ER membranes from HEK293T cells at a purity suitable for quantitative proteomics and lipidomics. Using cleavable, epitope‑tagged variants of SEC61β and REEP5, which preferentially localize to ER sheets and tubules, respectively, we isolate membrane vesicles corresponding to the two major ER subdomains. The proteomic analysis confirms the high purity of our preparation and the expected distribution of protein machineries: sheet‑associated factors, including translocon components and ribosomal proteins, are enriched in SEC61β isolates, whereas curvature‑stabilizing proteins are enriched in REEP5 isolates.
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-02_23:03:40.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-18 22:23:37	ID requested
⏵ 1	2026-09-02 23:03:41	announced

Dataset with its publication pending

submitter keyword: ER membrane composition, immunoisolation, mammalian cells,MemPrep, differential centrifugation

Robert Ernst
contact affiliation	Universität des Saarlandes
contact email	robert.ernst@uks.eu
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075836

PRIDE project URI

• PRIDE
  1. PXD075836
     1. Label: PRIDE project
     2. Name: Mammalian MemPrep establishes the lipid composition ER membranes in HEK293T cells