PXD075800

PXD075800 is an original dataset announced via ProteomeXchange.

Title	Photocatalytic Golgi Proteomics Reveals Palmitoylation-Regulated Golgiphagy
Description	The Golgi apparatus (GA) orchestrates protein modification, trafficking, and secretion through highly dynamic remodel-ing, yet its proteomic complexity remains difficult to resolve in living systems. Here, we report CAT-Golgi, a genetically independent and light-controlled photocatalytic proximity labeling strategy for in situ spatiotemporal mapping of the Golgi proteome. Combining a cysteine-conjugated eosin photocatalyst (GolgiCat) with an aniline probe, CAT-Golgi enables rapid and precise protein labeling within minutes under mild green light, requiring no genetic manipulation and operat-ing efficiently in hard-to-transfect and primary cells. Leveraging our extensive efforts in organelle-targeted photocatalytic systems, we extended this chemistry to the highly dynamic and reversible Golgi apparatus. CAT-Golgi achieved quantita-tive and comparative proteomics in HeLa, K562, Jurkat and primary HEKa cells, revealing both conserved and cell-type-specific profiles. Under Brefeldin A-induced Golgiphagy, CAT-Golgi captured large-scale proteome remodeling and identi-fied palmitoyl-protein thioesterase 1 (PPT1) as a central regulator. PPT1 downregulation enhanced ULK1 and TRPML1 palmitoylation, disrupted redox balance, and activated Golgiphagy. CAT-Golgi provides a broadly applicable chemical platform for decoding organelle dynamics, offering both conceptual and technical foundations for extending photocata-lytic proteomics to other transient organelles and illuminating molecular mechanisms of organelle plasticity and disease progression.
HostingRepository	iProX
AnnounceDate	2026-03-18
AnnouncementXML	Submission_2026-03-18_01:51:58.755.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yufei Di
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-18 01:51:29	ID requested
⏵ 1	2026-03-18 01:51:59	announced

Dataset with its publication pending

submitter keyword: Golgiphagy, Proximity labeling, Golgi apparatus

Xinyuan Fan
contact affiliation	Peking University
contact email	xinyuanfan@pku.edu.cn
lab head
Yufei Di
contact affiliation	Peking University
contact email	347247025@qq.com
dataset submitter

iProX dataset URI