PXD075792 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ubiquitylation by the GID/CTLH complex regulates the metabolic and innate immune response of macrophages to infection by Mycobacterium tuberculosis |
| Description | The GID/CTLH E3 ligase complex is implicated in several biological processes, yet its full substrate repertoire remains poorly defined. We recently identified the complex as a broad modulator of macrophage responses to Mycobacterium tuberculosis (Mtb) infection. Here, we use label-free proteomics and diGly capture analysis of Mtb-infected macrophages to define the GID/CTLH-dependent ubiquitylome. We identify thousands of dynamically altered ubiquitylation sites, with strong enrichment among proteins involved in cellular metabolism and innate immune signaling. Concurrent proteome analysis revealed extensive rewiring in GID/CTLH-deficient macrophages, with >90% of enriched pathways among increased proteins consisting of metabolic targets. Notably, inhibitory phosphatases (PTEN, INPP5D) also emerged as candidate substrates. Functional studies revealed proteasome-dependent stabilization of PTEN and INPP5D in GID/CTLH-deficient macrophages with each phosphatase individually exerting an influence on Mtb intracellular survival. Together, our study defines a GID/CTLH-dependent ubiquitylome in macrophages and identifies the complex as a central regulator of metabolism and antimicrobial immunity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-24 |
| AnnouncementXML | Submission_2026-06-24_00:31:34.811.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Nelson Simwela |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | autoflex |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-18 00:44:50 | ID requested | |
| ⏵ 1 | 2026-06-24 00:31:35 | announced | |
Publication List
Keyword List
| submitter keyword: metabolism, innate immunity, ubiquitylation, tuberculosis, GID/CTLH complex,Macrophages |
Contact List
| David G Russell |
|---|
| contact affiliation | Department of Microbiology & Immunology, Cornell University College of Veterinary Medicine |
| contact email | dgr8@cornell.edu |
| lab head | |
| Nelson Simwela |
|---|
| contact affiliation | Cornell University |
| contact email | nvs23@cornell.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075792
- Label: PRIDE project
- Name: Ubiquitylation by the GID/CTLH complex regulates the metabolic and innate immune response of macrophages to infection by Mycobacterium tuberculosis
