PXD075731

PXD075731 is an original dataset announced via ProteomeXchange.

Title	Secretomics data of Wnt2b+/+, Wnt2b-/- and Wnt2b-/- & MD-OV
Description	For each group, 3 mL of culture supernatant was collected in biological triplicates. The supernatants were then centrifuged at 3,000 xg to remove cell debris and filtered through a 0.22um filter. The filtered supernatants were immediately supplemented with a protease inhibitor cocktail (EDTA-free) to prevent degradation of secreted proteins. Protein concentration was performed using centrifugal ultrafiltration units with a molecular weight cut-off (MWCO) of 3 kDa or 10 kDa. Subsequently, four volumes of ice-cold acetone were added to the samples to precipitate the proteins. Protein samples were subjected to SDS-PAGE followed by silver staining to assess sample quality. Upon confirmation of adequate protein quality, the extracted proteins were digested using enzymes to generate peptides.
HostingRepository	iProX
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-17_00:42:58.170.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danqiong Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-17 00:42:40	ID requested
⏵ 1	2026-03-17 00:42:58	announced

Dataset with its publication pending

submitter keyword: Secretomics, WNT2B

Yang Cheng
contact affiliation	Guangzhou Women and Children's Medical Center
contact email	chengyang2872@sina.com
lab head
Danqiong Liu
contact affiliation	Guangzhou Medical University
contact email	25181219aa@sina.com
dataset submitter

iProX dataset URI