PXD075711

PXD075711 is an original dataset announced via ProteomeXchange.

Title	Magnolia officinalis (L.) Bark Extract Counteracts Oxidative Brain In-jury: A Proteomic Investigation into Neuroprotective Mechanisms
Description	Neurodegenerative diseases involve progressive neuronal loss associated with oxidative stress (OS) and inflammation. Given the limited efficacy of current therapies, natural compounds with multitarget neuroprotective potential are of growing interest. In this study, we investigated the neuroprotective effects of a standardized Magnolia officinalis (L.) bark extract (MOE), rich in honokiol and magnolol (29.1 ± 1.39 mg/g and 16.2 ± 0.81 mg/g, HPLC-UV DAD analysis) in rat brain cortical slices exposed to hydrogen peroxide-induced OS. MOE significantly recovered tissue viability and reduced ROS and malondialdehyde levels caused by OS. Caspase-3, -8, and -9 activation was also attenuated, suggesting a role in modulating both intrinsic and extrinsic apoptotic pathways. Shotgun proteomics using LC-HRMS/MS identified OS-induced protein expression changes reversed by MOE. Thirty-three proteins significantly altered by OS were identified, fourteen of which were rescued by MOE co-treatment. These proteins participate in several processes, including neuronal survival, OS response, and proteostasis. Bioinformatic analysis demonstrated that genes responsible for protein synthesis regulated by MOE are subjected to transcriptional regulation by factors associated with OS, including FOXO4, NRF2, and SP1. The present findings support the hypothesis that MOE exerts multitarget neuroprotective effects by modulating key proteins involved in OS responses and neuronal survival, suggesting a promising natural products-based strategy for pre-venting processes related to neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:14:41.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Stella
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-16 16:17:00	ID requested
⏵ 1	2026-05-24 16:14:42	announced

10.3390/ijms27083350;

Mattioli LB, Stella R, Peggion C, Cagnin S, Pifferi A, Miraldi E, Cappellucci G, Baini G, Camarda L, Budriesi R, Frosini M, (L.) Bark Extract Counteracts Oxidative Brain Injury: A Proteomic Investigation into Neuroprotective Mechanisms. Int J Mol Sci, 27(8):(2026) [pubmed]

submitter keyword: rat, label-free proteomics,Magnolia officinalis (L.), phytochemicals, age-related disor-ders, oxidative stress, neuroprotection, LC-HRMS/MS

Roberto Angeletti
contact affiliation	Istituto Zooprofilattico Sperimentale delle Venezie SCS2 - Dept. of Chemistry Italy
contact email	rangeletti@izsvenezie.it
lab head
Roberto Stella
contact affiliation	Istituto Zooprofilattico Sperimentale delle Venezie
contact email	rstella@izsvenezie.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075711
     1. Label: PRIDE project
     2. Name: Magnolia officinalis (L.) Bark Extract Counteracts Oxidative Brain In-jury: A Proteomic Investigation into Neuroprotective Mechanisms