PXD075617

PXD075617 is an original dataset announced via ProteomeXchange.

Title	Identification of p115 interacting proteins from HEK293T cells
Description	The protein p115 is a key player in the delivery of vesicles from the endoplasmic reticulum to the early Golgi. The protein is a homodimer with a folded head domain and a coiled-coil tail that is anchored to Golgi membranes. p115 has been shown to capture vesicles and to bind to SNARE proteins to promote membrane fusion. This project was to look for more interaction partners of p115 by expressing GFP fusions to the full length protein or to either the head or the tail alone. These fusions were expressed in HEK293T cells and co-precipitating proteins identified by mass-spectrometry and compared to those found with precipitation of GFP alone.
HostingRepository	PRIDE
AnnounceDate	2026-06-02
AnnouncementXML	Submission_2026-06-02_05:13:11.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina de Matos Ferraz Franco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-13 10:24:43	ID requested
⏵ 1	2026-06-02 05:13:12	announced

Yakunin I, Gillingham AK, Pereira C, Gershlick DC, Munro S, The Golgi vesicle tether p115/USO1 can bind directly to the ER exit site organiser Sec16A. J Cell Sci, ():(2026) [pubmed]

10.1242/jcs.264894;

submitter keyword: Human, LC-MSMS, p115, USO1, HEK293T, Sec16A

Sean Munro
contact affiliation	Division of Cell Biology MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH, UK
contact email	sean@mrc-lmb.cam.ac.uk
lab head
Catarina de Matos Ferraz Franco
contact affiliation	MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH
contact email	cffranco@mrclmb.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075617

PRIDE project URI

• PRIDE
  1. PXD075617
     1. Label: PRIDE project
     2. Name: Identification of p115 interacting proteins from HEK293T cells