PXD075613 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Photocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions |
| Description | Almost all cellular processes are influenced by ubiquitination. A large family of enzymes known as E3 ligases provide the specificity for ubiquitination, with the largest class among them, the RING E3s, comprising over 600 members in humans. RING E3s facilitate transfer of ubiquitin to substrates by constraining the highly dynamic E2-Ub thioester linkage to be primed for attack from the substrate nucleophile. We have established a workflow that uses a modified ubiquitin carrying a photoactivatable crosslinker that once stably linked to the active site of an E2, creates an activity based probe (ABP) to monitor interactions with E3 ligases. Using this, regions of interaction between ubiquitin and a selection of different RING E3 were determined, which not only confirmed existing structures of E2-Ub-RING-E3 complexes but was also used to assess new Ub-E2-E3 models generated in absence of existing structures. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-08_01:28:34.834.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mike Tatham |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | ubiquitinylated lysine; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-13 08:57:00 | ID requested | |
| ⏵ 1 | 2026-06-08 01:28:35 | announced | |
Publication List
| 10.1042/bcj20260213; |
| Chandler SF, Tatham MH, Branigan E, Nakasone MA, Makukhin N, Ciulli A, Hay RT, Photocross-linking activity-based probes to capture the dynamics of ubiquitin RING E3 ligase interactions. Biochem J, 483(7):1115-1130(2026) [pubmed] |
Keyword List
| submitter keyword: Ubiquitin N-maleimido-diazirine E3 Ligase Activity-based probe |
Contact List
| Ronald T. Hay |
|---|
| contact affiliation | Professor of Molecular Biology. University of Dundee. UK |
| contact email | r.t.hay@dundee.ac.uk |
| lab head | |
| Mike Tatham |
|---|
| contact affiliation | University of Dundee |
| contact email | m.tatham@dundee.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075613
- Label: PRIDE project
- Name: Photocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions
