PXD075602

PXD075602 is an original dataset announced via ProteomeXchange.

Title	Probing molecular diversity of brain cells with chemically modified aptamers
Description	We developed a volumetric correlated light and electron microscopy (CLEM) approach that uses slow off-rate modified aptamers (SOMAmers) for fluorescence labeling of cells in ultrastructurally reconstructable electron micrographs. The small size of aptamers enables intracellular labeling in fixed brain tissue without detergent permeabilization, thereby preserving tissue ultrastructure and allowing high-resolution electron microscopy imaging and accurate cellular segmentation. This method enables labeling of a wide range of molecular biomarkers, providing a powerful strategy to integrate molecular information with volumetric electron microscopy datasets. As a result, it facilitates molecularly annotated ultrastructural studies of complex tissues, including investigations of neural circuits. To ensure that the aptamers used in this method bind specifically to their intended protein targets, we validated target binding using pull-down mass spectrometry. These results were further confirmed through complementary approaches including genetic correlation analyses, proximity extension assays, and comparisons with commercial antibody labeling.
HostingRepository	PRIDE
AnnounceDate	2026-04-17
AnnouncementXML	Submission_2026-04-17_07:55:28.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaotang Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-13 02:28:10	ID requested
⏵ 1	2026-04-17 07:55:28	announced

10.1038/S41467-026-72180-7;

submitter keyword: neuroscience,pulldown MS

Xiaotang Lu
contact affiliation	University of Illinois Urbana-Champaign
contact email	xiaotang@illinois.edu
lab head
Xiaotang Lu
contact affiliation	University of Illinois Urbana-Champaign
contact email	xiaotang@illinois.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075602

PRIDE project URI

• PRIDE
  1. PXD075602
     1. Label: PRIDE project
     2. Name: Probing molecular diversity of brain cells with chemically modified aptamers