PXD075545

PXD075545 is an original dataset announced via ProteomeXchange.

Title	Title: Exploring the Effects of Scutellaria Baicalensis stem-leaf total flavonoids on Ulcerative Colitis Mice Based on Gut Microbiota and Proteomics
Description	Background: Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD). Scutellaria baicalensis Georgi (SBG), a widely used traditional Chinese medicine, exhibits anti-inflammatory and antioxidant properties. Scutellaria Baicalensis stem-leaf total flavonoids (SSTF) ameliorate colonic pathological damage in UC mouse model. However, its mechanism of action remains to be further explored. Aim of study: To investigate the therapeutic effects of SSTF on UC mice and the underlying mechanisms. Materials and methods: Construct a mouse model of UC and evaluate therapeutic efficacy by assessing clinical indicators and histopathological changes. Integrated gut microbiota and proteomics identified core targets associated with SSTF, and target validation was performed by immunohistochemistry. Results: Our results revealed that SSTF significantly improves clinical indicators (colon length, body weight, DAI score, spleen index, TNF-α, IL-10) in UC mice. Pathological damage was reduced, intestinal mucosal barrier impairment was improved, and core proteins (MUC2, ZO-1) were elevated. Proteomics identified 1116 differentially expressed genes. SSTF significantly regulated the intestinal flora structure of UC mice to promote the proliferation of beneficial bacterial genera. Three core targets (NR1D1, AMBRA1, YAP1) were validated by immunohistochemistry, confirming their roles in the treatment of UC with SSTF. Conclusion: SSTF improves UC and attenuates histopathological damage. These findings further elucidate the treatment mechanism of SSTF and provide new ideas for the treatment of UC.
HostingRepository	iProX
AnnounceDate	2026-03-11
AnnouncementXML	Submission_2026-03-12_01:45:34.506.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luwan Xing
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-12 01:45:17	ID requested
⏵ 1	2026-03-12 01:45:35	announced

Dataset with its publication pending

submitter keyword: Scutellaria baicalensis Georgi, Scutellaria Baicalensis stem-leaf total flavonoids, Ulcerative colitis, Proteomics, Gut microbiota

Fujiang Wang
contact affiliation	Suzhong Pharmaceutical R&D Institute Co., Ltd.
contact email	wangfj@suzhongyy.com
lab head
Luwan Xing
contact affiliation	China Pharmaceutical University
contact email	1519847109@qq.com
dataset submitter

iProX dataset URI