PXD075539

PXD075539 is an original dataset announced via ProteomeXchange.

Title	Cell type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
Description	Intercellular communication is critical for maintaining organismal metabolic homeostasis. Here, we present a new method enabling temporally controlled, cell type-specific labeling of secreted and membrane proteins in key metabolic tissues. The method employs a genetically encoded proximity-labeling strategy by targeting a Cre-dependent TurboID ligase to the endoplasmic reticulum (ER) in ES cell-derived mice. Expression of TurboID in liver, adipose tissue, and spleen enabled the characterization of organ-specific ER proteomes at baseline and in response to fasting, inflammation, and dietary obesity, revealing tissue- and perturbation-specific changes and augmenting our understanding of how the proteomes of individual tissues change to regulate systemic energy balance. This comprehensive resource represents an important advance toward understanding both how cell-to-cell communication changes in response to energy homeostasis and how it contributes to these alterations. This method is broadly applicable and provides a means for identifying biomarkers and therapeutic targets across a wide range of tissues.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_07:24:48.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henry Sanford
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-11 21:46:00	ID requested
⏵ 1	2026-05-13 07:24:49	announced

Dataset with its publication pending

submitter keyword: None

Ekaterina V. Vinogradova
contact affiliation	Laboratory of Chemical Immunology and Proteomics, Rockefeller University
contact email	vinograd@rockefeller.edu
lab head
Henry Sanford
contact affiliation	Rockefeller University
contact email	hsanford@rockefeller.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075539

PRIDE project URI

• PRIDE
  1. PXD075539
     1. Label: PRIDE project
     2. Name: Cell type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.