PXD075538

PXD075538 is an original dataset announced via ProteomeXchange.

Title	Deciphering NAC53, NAC78 interactome in response to Pst infection; NAC53, NAC78, MDP25 subcellular interactomes and identifying ubiquitination sites of NAC53 and NAC78 upon HRD1 E3 ligase mediated ubiquitination.
Description	Analysis of NAC53 and NAC78 interactome in normal condition or upon Pseudomonas syringea pv. tomato DC3000 ∆HopQ infection. Constructs promUBQ10::eGFP-NAC53/eGFP-NAC78 are used as baits and promUBQ10::eGFP is used as control. Analysis of ubiquitination sites on NAC53 and NAC78 upon HRD1a/b ubiquitination. Analysis of NAC53, NAC78 and MDP25 subcellular interactome. For NAC53 and NAC78, dual tagged constructs pLjUBQ1::mCerrulean-NAC53/78-mCherry were used to pull-down N-ter moieties (cytosolic/nuclear localization) or C-ter moieties (endoplasmic reticulum localization). pUBQ10::eGFP and p35S::mCherry-HDEL are used as controls. For MDP25, protein variants were used, MDP25FL (plasma membrane localization), MDP25∆N25 (cytosolic localization) or N25MDP25 (plasma membrane localization). pUBQ10::eGFP is used as control.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_06:28:35.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: NEWT:4100;
ModificationList	ubiquitinylated lysine; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-11 21:39:20	ID requested
⏵ 1	2026-03-23 06:28:35	announced

Dataset with its publication pending

submitter keyword: AP-MS,Interactome, ubiquitination, subcellular localization, bacterial infection, transcription factors, microtubule depolymerizing factor

Suayb Üstün
contact affiliation	Ruhr University Bochum Faculty of Biology and Biotechnology Plant Cell Biology
contact email	suayb.uestuen@ruhr-uni-bochum.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075538
     1. Label: PRIDE project
     2. Name: Deciphering NAC53, NAC78 interactome in response to Pst infection; NAC53, NAC78, MDP25 subcellular interactomes and identifying ubiquitination sites of NAC53 and NAC78 upon HRD1 E3 ligase mediated ubiquitination.