PXD075517

PXD075517 is an original dataset announced via ProteomeXchange.

Title	A proximity labelling derived luminal proteome of the Trypanosoma brucei endoplasmic reticulum, Golgi apparatus and nuclear membrane
Description	We employed proximity labelling to derive a proteome of the luminal content of the Trypanosoma brucei endoplasmic reticulum (ER) and Golgi apparatus. We exploited the abundant ER chaperone BiP (Binding-immunoglobulin protein) as ER BioID bait and compare to a truncated version of BiP (termed BiPN) devoid of ER retention and thus trafficked to the Golgi apparatus, as well as parental controls. Our approach covers the two major life cycle stages of T. brucei, i.e., the procyclic form (PCF) and the bloodstream form (BSF). Additionally, we probed the inner nuclear membrane which can be viewed as a specialised ER domain separated by the nuclear pore, via the membrane integral nucleoporin NUP65 as BioID bait in PCF. Altogether, our combined approaches devise an inventory of the T. brucei secretory pathway that complements existing subcellular protein maps derived by proteomics and imaging.
HostingRepository	PRIDE
AnnounceDate	2026-04-05
AnnouncementXML	Submission_2026-04-05_02:00:30.934.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-11 14:47:08	ID requested
⏵ 1	2026-04-05 02:00:31	announced

Dataset with its publication pending

submitter keyword: nuclear envelope, secretory pathway, endoplasmic reticulum, Golgi apparatus,Trypanosoma brucei

Martin Zoltner
contact affiliation	Head of Drug Discovery and Evaluation, Charles University in Prague, Department of Parasitology, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic
contact email	zoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075517
     1. Label: PRIDE project
     2. Name: A proximity labelling derived luminal proteome of the Trypanosoma brucei endoplasmic reticulum, Golgi apparatus and nuclear membrane