PXD075501

PXD075501 is an original dataset announced via ProteomeXchange.

Title	LC-MS of the VTC Complex BioID from Leishmania tarentolae
Description	Acidocalcisomes are evolutionarily conserved acidic organelles that are rich in cations and inorganic phosphate, primarily polyphosphates. In kinetoplastid parasites, acidocalcisomes and their polyphosphate content are essential for osmoregulation and environmental adaptation during host switching. In this organelle, polyphosphate is synthesised and transported to the lumen by the vacuolar transporter chaperone (VTC) complex. Interestingly, unlike yeast VTC, which has five components, only two have been observed in kinetoplastids: Vtc1, which contains only a transmembrane domain and Vtc4, which, in addition to a transmembrane domain, also consists of SPX and catalytic domains. In this study, we used proximity-dependent biotinylation (BioID) in Leishmania tarentolae to identify proteins located close to the VTC complex. The complex was found near several known acidocalcisomal proteins, including membrane-bound pyrophosphatase (mPPase), vacuolar-type H⁺-ATPase (V-H+-ATPase), Ca²⁺-transporting P-type ATPase (Ca2+-ATPase), zinc transporter (ZnT), and palmitoyl acyltransferase 2 (PAT2). Importantly, this approach revealed three novel VTC binding partners (VBPs) that colocalise and interact with the complex in acidocalcisomes, as confirmed by confocal microscopy, pulldown assays, and AlphaFold 3 structural predictions. Together, our results expand the acidocalcisome interactome and suggest that the newly identified VBPs may contribute to the structural organisation and regulatory function of the VTC complex in phosphate homeostasis of kinetoplastid parasites.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_09:59:23.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keni Vidilaseris
SpeciesList	scientific name: Leishmania tarentolae; NCBI TaxID: NEWT:5689;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-11 08:03:55	ID requested
⏵ 1	2026-05-21 09:59:24	announced

Dataset with its publication pending

submitter keyword: Proteome Discoverer 2.5, LC-MS,VTC complex, Leishmania tarentolae, VTC binding partners, acidocalcisomes, BioID

Keni Vidilaseris
contact affiliation	Department of Molecular and Integrative Biosciences University of Helsinki
contact email	keni.vidilaseris@helsinki.fi
lab head
Keni Vidilaseris
contact affiliation	University of Helsinki
contact email	keni.vidilaseris@helsinki.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075501

PRIDE project URI

• PRIDE
  1. PXD075501
     1. Label: PRIDE project
     2. Name: LC-MS of the VTC Complex BioID from Leishmania tarentolae