PXD075455

PXD075455 is an original dataset announced via ProteomeXchange.

Title	Succinyl-proteome profiling reveals HDAC8-mediated CAPZB desuccinylation regulates cytoskeleton remodeling in bleomycin-induced idiopathic pulmonary fibrosis
Description	Idiopathic pulmonary fibrosis (IPF) is a chronic progressive lung disease of unknown etiology, characterized by progressive scarring and fibrosis of the lungs, leading to irreversible loss of lung function. Current mechanism studies have found that fibroblasts, as the main effector cells, exhibit abnormal differentiation and proliferation in pulmonary fibrosis, thereby promoting collagen secretion for extracellular matrix (ECM) deposition. Cytoskeletal remodeling is the basis for fibroblast-to-myofibroblast differentiation, however, the key proteins and regulatory mechanisms involved in this process are still unclear. Here, F-actin-capping protein subunit beta (CAPZB) was identified to be desuccinylated at three lysine residues (K57, K95, and K235). Gain- and loss-of-function studies in vitro and in vivo demonstrated that CAPZB regulates cytoskeletal remodeling and differentiation of fibroblasts to myofibroblasts by capping the barbed end of F-actin, and mutation of the modification site confirmed the inhibition of CAPZB function by desuccinylation. Further studies showed that histone deacetylase enzymes 8 (HDAC8) could interacts with CAPZB, and its pro-fibrotic role as a desuccinylase was verified. Our findings suggested that desuccinylation inhibited CAPZB function and promoted cytoskeletal remodeling, targeting the succinylation modification of CAPZB could potentially serve as a novel therapeutic approach for pulmonary fibrosis.
HostingRepository	PRIDE
AnnounceDate	2026-04-30
AnnouncementXML	Submission_2026-04-30_07:20:28.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Du Guanglian
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	succinylated residue
Instrument	6220 Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-10 11:57:26	ID requested
⏵ 1	2026-04-30 07:20:29	announced

Liu B, Kang D, Zhang J, Wang Y, Li R, Lv C, Zhai N, Song X, Zhang S, Li H, HDAC8-mediated CAPZB desuccinylation enhances cytoskeleton remodeling to promote idiopathic pulmonary fibrosis. Commun Biol, ():(2026) [pubmed]

10.1038/s42003-026-10125-y;

submitter keyword: Idiopathic pulmonary fibrosis

Lysine succinylation

Succinyl-proteome

CAPZB

HDAC8

Cytoskeleton remodeling

LC-MS/MS

Mouse lung tissue

XiaoDong Song
contact affiliation	Binzhou Medical University
contact email	songxd71@bzmc.edu.cn
lab head
Du Guanglian
contact affiliation	Binzhou Medical University
contact email	d2435186757@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075455
     1. Label: PRIDE project
     2. Name: Succinyl-proteome profiling reveals HDAC8-mediated CAPZB desuccinylation regulates cytoskeleton remodeling in bleomycin-induced idiopathic pulmonary fibrosis