PXD075408

PXD075408 is an original dataset announced via ProteomeXchange.

Title	Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants
Description	Recognition of dsRNA triggers antiviral defense mediated by PKR and OAS3/RNase L pathways through translational arrest and RNA decay. This is accompanied by assembly of distinct cytoplasmic ribonucleoprotein (RNP) condensates termed stress granules (SGs) and RNase L-dependent bodies (RLBs). Here we show that adenovirus infection differentially modulates dsRNA sensors and RNP granule assembly. Infection with splicing-defective ∆E4 mutant leads to dsRNA accumulation and activation of both PKR and OAS3/RNase L, promoting formation of RLB-like granules. In contrast, mutants lacking virus-associated (VA) RNAs trigger PKR activation and assembly of SGs despite absence of detectable dsRNA. Proteomic analysis revealed distinct protein compositions of canonical SGs and RLBs, which were reflected in virus-induced granules. While ∆VA-induced granules were PKR-dependent, ∆E4 mutants induced RLB-like granules independently of PKR and RNase L. In these cells, granule assembly coincided with translational arrest independent of eIF2α phosphorylation, indicating additional pathways linking nuclear dsRNA sensing to translational control and RNP granule assembly during viral infection. These findings provide novel insights into how distinct dsRNA sensors modulate translation and RNP condensates in response to stress.
HostingRepository	PRIDE
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-07-10_11:58:34.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ORLANDO SCUDERO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-09 09:45:41	ID requested
⏵ 1	2026-07-10 11:58:35	announced

10.1371/JOURNAL.PPAT.1014452;

submitter keyword: DNA viruses, RNase L-dependent bodies (RLBs), Adenovirus 5 (Ad5),Stress Granules (SGs)

Benjamin A. Garcia
contact affiliation	Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, Missouri, USA
contact email	bagarcia@wustl.edu
lab head
ORLANDO SCUDERO
contact affiliation	Children's Hospital of Philadelphia
contact email	scuderoo@chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD075408

PRIDE project URI

• PRIDE
  1. PXD075408
     1. Label: PRIDE project
     2. Name: Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants