PXD075381

PXD075381 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of BAR against placental dysfunction in RU486-induced abortion-prone mice
Description	Background Recurrent spontaneous abortion (RSA) remains a formidable clinical challenge with limited therapeutic options, largely attributed to trophoblast dysfunction as a central pathological mechanism. Bushen Antai recipe (BAR) has demonstrated encouraging clinical and experimental efficacy in pregnancy support; however, its bioactive constituents and molecular mechanisms, especially in the context of trophoblasts, remain poorly understood. Purpose To evaluate the therapeutic efficacy of BAR in RSA and elucidate its underlying mechanisms, with a particular focus on trophoblast function, using an integrated strategy. Methods UPLC-Q-TOF-MS/MS was employed to profile chemical constituents of BAR and its serum-absorbed components. An RU486-induced abortion-prone mouse model and HTR-8/SVneo trophoblast injury model were established to evaluate BAR's effects on embryonic development and placentation. Label-free quantitative proteomics, network pharmacology, molecular docking, viral transduction, together with transmission electron microscopy imaging, morphological analysis, western blot, immunofluorescence, and various cellular functional assays were performed to decipher the underlying mechanisms. Results BAR significantly reduced embryo resorption, restored placental architecture, and improved trophoblast survival and function in abortion-prone mice. Proteomics revealed endoplasmic reticulum (ER) stress as a central pathological feature of RSA placentas, which was effectively ameliorated by BAR treatment. Network pharmacology predicted VEGFA as a core target, subsequently validated by experiments demonstrating that BAR activates the VEGFA/p-AKT/SESN2 axis to reprogram the dynamics of all three unfolded protein response (UPR) branches and re-establish ER homeostasis. Serum pharmacochemistry identified 72 absorbed constituents, with molecular docking revealing that multiple compounds including quercetin, calycosin, loganin, and sweroside may directly target VEGFA.
HostingRepository	iProX
AnnounceDate	2026-03-07
AnnouncementXML	Submission_2026-03-09_01:38:11.110.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-09 01:37:57	ID requested
⏵ 1	2026-03-09 01:38:11	announced

Dataset with its publication pending

submitter keyword: Recurrent spontaneous abortion, Bushen Antai recipe,Trophoblasts, Endoplasmic reticulum stress

Yufan Song
contact affiliation	Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	songyufan23@163.com
lab head
Zhuo Liu
contact affiliation	Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	zhuoliutjzx@126.com
dataset submitter

iProX dataset URI