PXD075307 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomic profiling of kinobead-enriched fractions from LF-268-treated HCT-116 cells via SILAC-based quantification |
| Description | SILAC-based phosphoproteomic profiling of kinase-enriched fractions from HCT-116 cells treated with the DFG-out pan-RAF inhibitor LF-268 to characterize kinase signaling responses. Cells were metabolically labeled and treated with 1 µM LF-268 or DMSO for 1 hour, followed by serial kinobead enrichment and Fe³⁺-NTA IMAC phosphopeptide enrichment prior to LC-MS/MS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-20 |
| AnnouncementXML | Submission_2026-04-20_08:35:25.495.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel Brush |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-06 00:01:13 | ID requested | |
| ⏵ 1 | 2026-04-20 08:35:26 | announced | |
Publication List
Keyword List
| submitter keyword: LC-MSMS,Phosphoproteomics, Human, Kinase, DDA |
Contact List
| Dustin James Maly |
|---|
| contact affiliation | Department of Chemistry, University of Washington |
| contact email | djmaly@uw.edu |
| lab head | |
| Daniel Brush |
|---|
| contact affiliation | University of Washington |
| contact email | dbrush@uw.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075307 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075307
- Label: PRIDE project
- Name: Phosphoproteomic profiling of kinobead-enriched fractions from LF-268-treated HCT-116 cells via SILAC-based quantification
