PXD075293 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Macrophage Proteomic Profiling Reveals Divergent TLR4-Dependent and -Independent Responses to Kdo₂-Lipid A and Lipid IVa |
| Description | Immortalized mouse macrophages from wild type (WT) were treated with Kdo₂-Lipid A and Lipid IVa under three experimental conditions: (i) extracellular stimulation to assess TLR4-dependent activation with Kdo2 lipid A or lipid-IVa (Extra_Kdo2/Extra_IVa), (ii) direct cytosolic delivery by transfection to evaluate TLR4-independent sensing with Kdo2 lipidA and lipid IVa (Intra_Kdo2/Intra_IVa), and (iii) double activation consisting of priming with Pam3CSK4 (P3C) for 6 h followed by transfection with either Kdo2 or IVa for 24 h to induce non-canonical inflammasome activation (DA_Kdo2/DA_IVa). We also use extracellular P3C stimulation for 6h for another control of priming. Untreated macrophges were serve as a control. This project also contains the pilot study data of Acyloxyacyl hydrolase (AOAH) knockout under non-canonical inflammasome activation (KO_DA_Kdo2/KO_DA_IVa). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-20 |
| AnnouncementXML | Submission_2026-08-20_04:08:35.267.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Aleksandra Nita-Lazar |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | carbamoylated residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-05 18:48:45 | ID requested | |
| ⏵ 1 | 2026-08-20 04:08:36 | announced | |
Publication List
| 10.3390/life16050753; |
| Issara-Amphorn J, Schoonmaker JL, Bradfield C, Yoon SH, Fraser IDC, Nita-Lazar A, -Lipid A and Lipid IVa. Life (Basel), 16(5):(2026) [pubmed] |
Keyword List
| submitter keyword: Kdo2-Lipid A, Lipid Iva, TLR4-independent,Macrophages, TLR4-dependent, Proteomics |
Contact List
| Aleksandra Nita-Lazar |
|---|
| contact affiliation | Functional Cellular Networks Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1892, USA |
| contact email | nitalazarau@niaid.nih.gov |
| lab head | |
| Aleksandra Nita-Lazar |
|---|
| contact affiliation | NIAID |
| contact email | nitalazarau@niaid.nih.gov |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075293
- Label: PRIDE project
- Name: Macrophage Proteomic Profiling Reveals Divergent TLR4-Dependent and -Independent Responses to Kdo₂-Lipid A and Lipid IVa
