PXD075274

PXD075274 is an original dataset announced via ProteomeXchange.

Title	Widespread Cytoskeletal Engagement of T. brucei KMP11
Description	KMP11 is an essential and highly conserved kinetoplastid protein. Using immunofluorescence, ultrastructure expansion microscopy and pulldown experiments, we show that KMP11 localizations in Trypanosoma brucei include the flagellum attachment zone, the paraflagellar rod, the flagellar connector, the microtubule quartet and the central alpha-helical domain of the tripartite attachment complex subunit p197. However, unlike previously reported, KMP11 does not localize to the basal body. Expansion microscopy and pulldown experiments furthermore showed that T. brucei KMP11 also binds to the central domain of T. cruzi p197. The central domains of p197 from the two species do not share a sequence homology but both have a high alpha-helical content. Thus, KMP11 may not bind to a specific peptide but to a common structural element of alpha-helices. KMP11 forms a rod-shaped homodimer which would be consistent with a crosslinking function. Thus, we speculate that KMP11 forms bundles between p197 molecules, and between other cytoskeletal proteins with a high alpha-helical content, offering a unifying model for its widespread localization.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_04:00:11.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Bender
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-05 08:34:13	ID requested
⏵ 1	2026-08-17 04:00:11	announced

10.1242/JCS.264898;

submitter keyword: Trypanosoma brucei, proteomics, affinity purification-mass spectrometry, p197,SILAC, KMP11

Bettina Warscheid
contact affiliation	Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany
contact email	bettina.warscheid@uni-wuerzburg.de
lab head
Julian Bender
contact affiliation	University of Wuerzburg Chair of Biochemistry II Am Hubland 97074 Würzburg
contact email	julian.bender@uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075274
     1. Label: PRIDE project
     2. Name: Widespread Cytoskeletal Engagement of T. brucei KMP11