PXD075270

PXD075270 is an original dataset announced via ProteomeXchange.

Title	PROTEOGOLD: Generation of Reference MS/MS Datasets for Large Protein Using FTMS Simulation
Description	Top- and middle-down (TD/MD) mass spectrometry is widely used to characterize proteins and biotherapeutics. These workflows routinely produce highly complex, information-dense spectra—overlapping isotopic patterns, multiple charge-state envelopes, and crowded product-ion populations—that increasingly challenge existing data processing and interpretation tools. Robust evaluation of such tools, however, is hindered by the limited availability of “gold-standard” datasets that capture realistic experimental complexity across instruments, acquisition conditions, and analyte classes while providing known ground truth. Here we present simulated TD-MS/MS spectra for carbonic anhydrase II generated within the ProteoGold dataset initiative. By producing in-silico spectra under defined conditions, ProteoGold provides extensible reference datasets designed for benchmarking, education, and method development in TD/MD MS.
HostingRepository	PRIDE
AnnounceDate	2026-05-26
AnnouncementXML	Submission_2026-05-26_02:46:33.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nina Khristenko
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	acetylated residue
Instrument	fourier transform ion cyclotron resonance; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-05 06:40:37	ID requested
⏵ 1	2026-05-26 02:46:33	announced

10.26434/CHEMRXIV.15003684;

submitter keyword: None

Yury O.
contact affiliation	Spectroswiss, Switzerland
contact email	tsybin@spectroswiss.ch
lab head
Nina Khristenko
contact affiliation	Spectrotech
contact email	khristenko.nina@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075270

PRIDE project URI

• PRIDE
  1. PXD075270
     1. Label: PRIDE project
     2. Name: PROTEOGOLD: Generation of Reference MS/MS Datasets for Large Protein Using FTMS Simulation