PXD075262

PXD075262 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Bifemetstrobin against the tea anthracnose pathogen
Description	Tea anthracnose caused by Glomerella cingulata threatens yield and quality, while resistance to legacy fungicides highlights the need for new modes of action. We characterized the antifungal activity and mechanism of bifemetstrobin against G. cingulata by combining efficacy assays with cell biology and integrated multi‑omics. Bifemetstrobin inhibited mycelial growth (EC50 = 5.04 μg/mL) and spore germination and provided up to approximately 80% curative suppression on detached tea leaves. Microscopy revealed dose‑dependent hyphal and conidial deformation and mitochondrial injury. Consistently, bifemetstrobin collapsed mitochondrial membrane potential (MMP), reduced intracellular ATP, and increased membrane leakage with depletion of reducing sugars and soluble proteins. Transcriptome, data-independent acquisition (DIA) proteomics and metabolome data converged on reprogramming of carbohydrate and amino‑acid metabolism, membrane processes, and redox homeostasis, with DNA‑replication signatures emerging at the higher dose. Cross-layer mapping prioritized an amine oxidase (AO) as a hub. Bifemetstrobin bound AO with micromolar affinity (microscale thermophoresis/isothermal titration calorimetry), supported by docking and molecular dynamics, and outperformed azoxystrobin in binding. Pairing bifemetstrobin with pyrrolnitrin yielded strong in vitro synergy and improved leaf protection. Across additional phytopathogens, bifemetstrobin retained activity in vitro (EC50 range 1.19–27.38 μg/mL), outperforming azoxystrobin on the tea anthracnose strain. These findings nominate AO as a high‑confidence candidate supported by binding assays and link target engagement to mitochondrial failure and carbon-nitrogen reprogramming, supporting bifemetstrobin (alone or in rational mixtures) as a promising candidate for tea anthracnose management.
HostingRepository	iProX
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_01:11:45.930.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinpeng Zhang
SpeciesList	scientific name: Colletotrichum gloeosporioides f. sp. camelliae; NCBI TaxID: 1215364;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-05 01:11:26	ID requested
⏵ 1	2026-03-05 01:11:46	announced

Dataset with its publication pending

submitter keyword: Proteomic, Bifemetstrobin

Xinpeng Zhang
contact affiliation	Guizhou University
contact email	zhangxinpeng175@163.com
lab head
Xinpeng Zhang
contact affiliation	Guizhou University
contact email	zhangxinpeng175@163.com
dataset submitter

iProX dataset URI