PXD075250

PXD075250 is an original dataset announced via ProteomeXchange.

Title	Dibutyl phthalate inhibits chondrocytes proliferation, promotes apoptosis and pyroptosis of chondrocytes to aggravate osteoarthritis progression by activating NF-κB signaling
Description	Dibutyl phthalate (DBP) is widely used as plasticizer in numerous kinds of products and migrate into the human bodies through food plastic packaging, posing potential health hazards to organisms. Accumulating experimental evidence has shown that DBP causes multiple organ damage, such as aggravate the allergic airway inflammation and mediate oxidative stress induces splenic injury in mice. However, the specific toxicity and underlying mechanism of DBP-induced knee joint injury remains poorly understood, especially any association between DBP exposure with pathological progression of osteoarthritis (OA) remain elusive. In our study, mice were treated by intragastric administration of DBP (50 mg/kg or 250 mg/kg) five times per week for 4 and 8 weeks, and results suggested that DBP exposure accelerated the regression of OA. Specifically, DBP augmented cartilage matrix degradation by decreasing Col2, Aggrecan and increasing Mmp3, Mmp13 and Adamts5. Further analysis revealed that DBP significantly inhibited chondrocytes proliferation, promoted apoptosis and pyroptosis of chondrocytes, augmented sensory nerve ingrowth in subchondral bone. Mechanistical analysis revealed that DBP stimulated NF-κB signaling by increasing I-κBα phosphorylation and subsequent p65 nuclear translocation. The data suggest that DBP inhibits chondrocyte proliferation, promotes apoptosis and pyroptosis of chondrocytes to accelerate the progression of OA by activating NF-κB signaling. In summary, our data provides a new insight into the impacts of the DBP-induced chondrocyte toxicity as well as the safety evaluation of DBP.
HostingRepository	iProX
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-04_19:34:32.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiguo Zhang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-04 19:34:16	ID requested
⏵ 1	2026-03-04 19:34:32	announced

Dataset with its publication pending

submitter keyword: Dibutyl phthalate (DBP), Osteoarthritis, Articular cartilage, Apoptosis, Pyroptosis, NF-κB signaling

Hongfeng Ruan
contact affiliation	Institute of Orthopaedics and Traumatology, the First Affiliated Hospital of Zhejiang Chinese Medical University
contact email	rhf@zcmu.edu.cn
lab head
Zhiguo Zhang
contact affiliation	Institute of Orthopaedics and Traumatology, the First Affiliated Hospital of Zhejiang Chinese Medical University
contact email	202311020811065@zcmu.edu.cn
dataset submitter

iProX dataset URI