PXD075227

PXD075227 is an original dataset announced via ProteomeXchange.

Title	UVPD of peptides modified with N-terminal UV-absorbing chromophores
Description	Ultraviolet photodissociation (UVPD) has emerged as a powerful alternative to conventional collision-induced dissociation (CID) for peptide and protein sequencing in mass spectrometry–based proteomics. However, UVPD efficiency depends on the presence of UV-absorbing chromophores within analytes.The uploaded dataset is a systematic investigation of how N-terminal modification of peptides with aromatic chromophores influences 193 nm UVPD efficiency and fragmentation behavior. Using NHS ester chemistry, human cell lysate digests were derivatized with a range of UV-absorbing aromatic labels and analyzed on an Omnitrap–Orbitrap platform equipped with a 193 nm ArF excimer laser. Compared with unmodified controls, derivatized peptides displayed modestly increased fragmentation efficiency and greater diversity of sequence-informative fragments, particularly b-type ions. Peptides lacking aromatic amino acids benefited most from N-terminal aromatic labeling, exhibiting enhanced fragmentation and sequence coverage. These findings demonstrate that incorporation of aromatic chromophores at the N-terminus can enhance UVPD performance and improve spectral interpretability, thereby expanding the applicability of UVPD in bottom-up proteomics workflows.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:09:19.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikita Levin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-04 12:36:09	ID requested
⏵ 1	2026-06-07 16:09:20	announced

Levin N, Demyanenko Y, Kitano E, Mohammed S, -Terminal UV-Absorbing Chromophores. J Am Soc Mass Spectrom, 37(5):1124-1131(2026) [pubmed]

10.1021/jasms.5c00389;

submitter keyword: chromophores,LC-MS, proteomics, NHS chemistry, UVPD

Shabaz Mohammed
contact affiliation	The Rosalind Franklin Institute; University of Oxford, Department of Biochemistry; University of Oxford, Department of Chemistry
contact email	shabaz.mohammed@chem.ox.ac.uk
lab head
Nikita Levin
contact affiliation	University of Oxford
contact email	nikita.levin@rfi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075227

PRIDE project URI

• PRIDE
  1. PXD075227
     1. Label: PRIDE project
     2. Name: UVPD of peptides modified with N-terminal UV-absorbing chromophores