PXD075214

PXD075214 is an original dataset announced via ProteomeXchange.

Title	Yellow protein co-opted to sustain obligate symbiosis in leaf beetles
Description	Yellow proteins are best known for their roles in pigmentation, behavior, and development across insects. Here, we uncover their striking evolutionary co-option for a wholly distinct function: sustaining a Paleocene-aged digestive symbiosis in tortoise beetles. We show that a female-specific Yellow forms the gelatinous spheres that encapsulate the bacterium Stammera during vertical transmission, allowing it to subsist extracellularly despite its drastically reduced genome (0.24 Mb) and limited metabolic capacity. Yellow expression is highly localized to symbiont-harboring glands in the ovaries, where the protein is assembled into a matrix and secreted during egg-laying. Functional knockdown of yellow disrupts sphere integrity and compromises symbiont viability under dry conditions, underscoring the protein’s embedding properties and protective role for Stammera. These findings reveal an unreported function for an ancient gene family and demonstrate how tortoise beetles have repurposed Yellows to overcome the extreme metabolic constraints faced by their symbionts during extracellular transmission.
HostingRepository	PRIDE
AnnounceDate	2026-06-16
AnnouncementXML	Submission_2026-06-16_06:40:17.924.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Velic
SpeciesList	scientific name: Acromis sparsa; NCBI TaxID: NEWT:111205; scientific name: Charidotella sexpunctata; NCBI TaxID: NEWT:111225; scientific name: Chelymorpha gressoria; NCBI TaxID: NEWT:325176; scientific name: Aspidimorpha; NCBI TaxID: NEWT:1454958; scientific name: Cassida rubiginosa; NCBI TaxID: NEWT:294607;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-04 09:05:48	ID requested
⏵ 1	2026-06-16 06:40:18	announced

Dataset with its publication pending

submitter keyword: Symbiosis

yellow proteins

LC-MS

Hassan Salem
contact affiliation	Mutualisms Research Group, Max Planck Institute for Biology, Tübingen 72076, Germany Department of Molecular Microbiology, John Innes Centre, Norwich, UK
contact email	hassan.salem@jic.ac.uk
lab head
Ana Velic
contact affiliation	University of Tuebingen
contact email	a.velic@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075214

PRIDE project URI

• PRIDE
  1. PXD075214
     1. Label: PRIDE project
     2. Name: Yellow protein co-opted to sustain obligate symbiosis in leaf beetles