PXD075203

PXD075203 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry data for PAL-catalyzed peptide cyclization and affibody–peptide ligation
Description	This dataset contains mass spectrometry measurements acquired to verify peptide products generated by a peptide asparaginyl ligase (PAL)-catalyzed reaction, including (i) peptide substrate cyclization and hydrolysis side product formation, and (ii) protein–peptide ligation. For cyclization, the linear peptide GL12 (GIARGDYKSNSL) was processed by PAL to yield the cyclic product cGN10 (GIARGDYKSN, head-to-tail cyclized) and a linear hydrolysis by-product GN10 (GIARGDYKSN). For ligation, an affibody protein (ABY) was conjugated at its C-terminus to a short fluorescent/azide-containing peptide FL (GWSK(5-FAM)K(N3)-NH2), generating the ligation product ABY–FL. Raw MS files and deconvoluted peak lists/summaries are provided for intact mass confirmation of reactants and products.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:10:33.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shi Qi
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-04 03:59:14	ID requested
⏵ 1	2026-08-23 16:10:34	announced

Du W, Qi S, Zhen M, Liao M, Zhao Y, Ma J, Hao Y, Jiang H, Lu G, Liew CW, Chua N, Chen H, Hu G, Hemu X, Mining of natural diversity enables efficient and expressible peptide asparaginyl ligases. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-72367-y;

submitter keyword: transpeptidation, affibody, peptide cyclization, bioconjugation, intact mass spectrometry,peptide asparaginyl ligase

Xinya Hemu
contact affiliation	School of Traditional Chinese Pharmacy, China Pharmaceutical University, China
contact email	hemuxinya@cpu.edu.cn
lab head
Shi Qi
contact affiliation	China Pharmaceutical University
contact email	983493573@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD075203

PRIDE project URI

• PRIDE
  1. PXD075203
     1. Label: PRIDE project
     2. Name: Mass spectrometry data for PAL-catalyzed peptide cyclization and affibody–peptide ligation