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PXD075185
PXD075185 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lactate-p300 activates macrophage-fibroblast-like synoviocytes communication via H3K18la to promote rheumatoid arthritis |
| Description | This study investigated the role of lactate‑induced histone H3K18 lactylation (H3K18la) in the pathology of rheumatoid arthritis (RA).Immunohistochemistry, western blot, gene knockdown/overexpression, proteomics, transwell co‑culture, and flow cytometry were employed to explore the function and mechanism of H3K18la in RA.Results demonstrated that elevated lactate levels in M1 macrophages promote H3K18la through p300, leading to the upregulation of METTL16 in these macrophages.When co‑cultured with M1 macrophages, fibroblast‑like synoviocytes (FLS) showed enhanced Wnt/β‑catenin signaling (increased expression of Wnt1) and elevated levels of pro‑invasive markers including MMP3 and fibronectin. These effects were abolished by p300 knockdown in macrophages, but restored by enforced overexpression of METTL16.In conclusion, lactate‑p300‑mediated H3K18la in M1 macrophages drives RA progression via METTL16/Wnt1‑dependent crosstalk with FLS, linking lactate metabolism and histone lactylation in M1 macrophages to the pathogenesis of RA. |
| HostingRepository | iProX |
| AnnounceDate | 2026-03-04 |
| AnnouncementXML | Submission_2026-03-03_23:10:59.060.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yingqiu Song |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-03 23:10:43 | ID requested | |
| ⏵ 1 | 2026-03-03 23:10:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Rheumatoid arthritis, Lactate, p300, H3K18la, Macrophage-FLS communication |
Contact List
| Chenggui Miao | |
|---|---|
| contact affiliation | Anhui University of Chinese Medcine |
| contact email | miaocg@ahtcm.edu.cn |
| lab head | |
| Yingqiu Song | |
| contact affiliation | Anhui University of Chinese Medcine |
| contact email | s202320422104@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
