⮝ Full datasets listing
PXD075157
PXD075157 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics of iPSC derived enteric neural lineages from Parkinson Disease patients |
| Description | Gastrointestinal (GI) dysfunction precedes motor symptoms in Parkinson disease (PD), implicating the enteric nervous system (ENS) in early disease pathogenesis. However, how the PD-associated protein alpha synuclein (alpha syn) contributes to ENS dysfunction, and whether this is influenced by inflammation, remains unresolved. Here, we show that tumor necrosis factor alpha (TNF alpha) increases alpha syn accumulation at mitochondria, disrupts the malate-aspartate shuttle (MAS), and induces a metabolic shift toward glutamine oxidation in iPSC derived enteric neural lineages (ENLs) from PD patients carrying alpha syn gene triplications. This metabolic rewiring leads to mitochondrial dysfunction, NAD+ depletion, and oxidative stress. Targeting glutamate metabolism with Chicago Sky Blue 6B restores mitochondrial function and reverses TNF alpha induced metabolic impairment. Combined transcriptomic and histological analyses of human gut tissue show that inflammation-associated MAS suppression and alpha syn upregulation are not confined to PD but are general hallmarks of intestinal inflammation. These findings highlight a conserved metabolic vulnerability in the ENS and establish iPSC ENLs as a powerful platform for modeling early inflammatory disease mechanisms. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-03 |
| AnnouncementXML | Submission_2026-03-03_11:46:56.411.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bruno |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-03 11:37:46 | ID requested | |
| ⏵ 1 | 2026-03-03 11:46:56 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: iPSC, Parkinson, Enteric Nervous System, DatasetType:Proteomics |
Contact List
| Beate Winner | |
|---|---|
| contact affiliation | Friedrich Alexander Universitat Erlangen Nurnberg |
| contact email | beate.winner@fau.de |
| lab head | |
| Bruno | |
| contact affiliation | Friedrich-Alexander Universitat Erlangen-Nurnberg |
| contact email | brghirotto@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098371/ |
