PXD075155

PXD075155 is an original dataset announced via ProteomeXchange.

Title	MS/MS analysis of in-gel tryptic peptides of wild-type and mutants of glutamine amidotransferase from Methanocaldococcus jannaschii
Description	A stable post-translational modification, succinimide (SNN), found in Methanocaldococcus jannaschii (Mj) glutamine amidotransferase (GATase) is shown to impart hyper-thermostability to the protein. To explore the role of neighbouring residues in enabling deamidation of Asn109 to SNN, intact protein mass analysis on 12 mutants of MjGATase was performed. Among these mutants, 8 showed varied proportions of mass corresponding to unmodified Asn109. SNN is known to undergo rapid hydrolysis to Asp/iso-Asp, hence, this population can also exist. As Asn intact and Asp/iso-Asp populations differ by only 1 Da, ambiguity exists in their assignment. Therefore, to determine the true levels of unmodified Asn109 population, in-gel trypsin digestion followed by MS/MS analysis of the tryptic peptides for 8 mutants and wild-type of MjGATase was conducted. The MS/MS analysis of two double mutants assured that they indeed retain Asn109 intact, forming a major population. This study points toward the presence of internal catalysts that enable the deamidation of Asn109 to SNN in MjGATase.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-08_01:28:01.632.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD075155
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hemalatha Balaram
SpeciesList	scientific name: Methanocaldococcus jannaschii; NCBI TaxID: NEWT:2190;
ModificationList	deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-03 10:58:06	ID requested
⏵ 1	2026-06-08 01:28:02	announced

10.6019/PXD075155;

Chandrashekarmath A, Jash O, Singh K, Bellur A, Roy CS, Dongre A, Chathoth NE, Anjukandi P, Kumar S, Mukherjee S, Balaram P, Balasubramanian S, Balaram H, Insights into the role of internal catalysts in asparagine-to-succinimide conversion in a hyperthermophilic glutamine amidotransferase. J Biol Chem, 302(6):113093(2026) [pubmed]

10.1016/j.jbc.2026.113093;

submitter keyword: LC-MS/MS, succinimide, deamidation

Hemalatha Balaram
contact affiliation	Molecular Biology and Genetics Unit, Jawaharlal Nehru Centrefor Advanced Scientific Research, Bengaluru, India
contact email	hb@jncasr.ac.in
lab head
Hemalatha Balaram
contact affiliation	Professor
contact email	hb@jncasr.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075155
     1. Label: PRIDE project
     2. Name: MS/MS analysis of in-gel tryptic peptides of wild-type and mutants of glutamine amidotransferase from Methanocaldococcus jannaschii