PXD075103

PXD075103 is an original dataset announced via ProteomeXchange.

Title	Peptidomics-guided identification of TRPV1-targeting peptides from Apis mellifera venom and macrophage proteomics
Description	Bee venom has been traditionally used in the treatment of inflammatory disorders and is known to contain diverse bioactive peptide components. In this study, we systematically characterized the Apis mellifera venom peptidome and investigated peptides enriched under TRPV1 pull-down conditions with potential inflammatory modulatory activity. Crude venom was fractionated by AKTA chromatography, and individual fractions were evaluated by SDS–PAGE. Protein bands were subjected to in-gel digestion followed by LC–MS/MS protein identification. Fraction F4, which displayed a prominent A280 absorbance peak during AKTA chromatography, was further analyzed by LC–MS/MS–based peptidomics. De novo sequencing results from fraction F4 were used to construct an in-house custom peptide database. Two independent GST pull-down workflows (exploratory and in-house) were performed using GST-TRPV1 and GST controls. Candidate peptides enriched in the GST-TRPV1 pull-down relative to GST controls were identified following background subtraction and peak-area filtering criteria. Selected synthetic peptide candidates were subsequently evaluated using macrophage-based proteomics experiments to assess their potential inflammatory modulatory effects. The deposited dataset includes raw LC–MS/MS files and processed outputs for gel-based protein identification, venom peptidomics, pull-down–associated peptide identification, macrophage proteomics (MaxQuant analysis), and the custom venom peptide FASTA library used for database searches.
HostingRepository	PRIDE
AnnounceDate	2026-04-17
AnnouncementXML	Submission_2026-04-17_00:55:38.873.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Wang
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-02 12:16:18	ID requested
⏵ 1	2026-04-17 00:55:39	announced

10.1021/ACS.JNATPROD.5C01554;

submitter keyword: peptidomics, proteomics, macrophage, inflammation,Apis mellifera venom, pull-down, TRPV1

Hongcheng Zhang
contact affiliation	State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
contact email	460414874@qq.com
lab head
Kai Wang
contact affiliation	University of Liège; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
contact email	1029020173@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075103
     1. Label: PRIDE project
     2. Name: Peptidomics-guided identification of TRPV1-targeting peptides from Apis mellifera venom and macrophage proteomics