PXD075095

PXD075095 is an original dataset announced via ProteomeXchange.

Title	Intact protein mass analysis of wild-type and mutants of glutamine amidotransferase from Methanocaldococcus jannaschii
Description	The glutamine amidotransferase (GATase) from Methanocaldococcus jannaschii (Mj) harbours a stable post-translational modification, succinimide (SNN). As shown from earlier studies, the spontaneous deamidation of Asn109 leading to the formation of stable SNN in MjGATase, imparts hyper-thermostability to the protein. To examine which of the residues in the environment of Asn109 catalyses the conversion of Asn to SNN, several mutants of MjGATase were generated. The intact protein masses of these mutants along with the wild-type enzyme were obtained through mass spectrometric analysis. The wild-type enzyme shows a mass difference of 17 Da due to the formation of SNN whereas, the MjGATase mutants harboring unmodified Asn109 would retain the mass corresponding to that obtained from the sequence. The mass analysis of 12 MjGATase mutant proteins showed varied levels retaining intact Asn109 residue. Two double mutants showed almost entire population with the expected mass, thus, highlighting the involvement of the neighbouring residues in formation of SNN.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:09:13.584.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hemalatha Balaram
SpeciesList	scientific name: Methanocaldococcus jannaschii; NCBI TaxID: NEWT:2190;
ModificationList	deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-02 10:49:13	ID requested
⏵ 1	2026-06-07 16:09:14	announced

Chandrashekarmath A, Jash O, Singh K, Bellur A, Roy CS, Dongre A, Chathoth NE, Anjukandi P, Kumar S, Mukherjee S, Balaram P, Balasubramanian S, Balaram H, Insights into the role of internal catalysts in asparagine-to-succinimide conversion in a hyperthermophilic glutamine amidotransferase. J Biol Chem, 302(6):113093(2026) [pubmed]

10.1016/j.jbc.2026.113093;

submitter keyword: LC-MS, succinimide, deamidation

Hemalatha Balaram
contact affiliation	Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific research, Bengaluru, India
contact email	hb@jncasr.ac.in
lab head
Hemalatha Balaram
contact affiliation	Professor
contact email	hb@jncasr.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075095
     1. Label: PRIDE project
     2. Name: Intact protein mass analysis of wild-type and mutants of glutamine amidotransferase from Methanocaldococcus jannaschii