⮝ Full datasets listing
PXD075077
PXD075077 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | IP-MS raw data of proteins precipitated by Mpro-Flag and the phosphorylation site of Mpro in 293T-Mpro-Flag cells |
| Description | The main protease (Mpro, also known as 3CLpro) of SARS-CoV-2 plays a pivotal role in the viral life cycle by mediating the cleavage of viral polyproteins. Understanding the regulatory mechanisms of Mpro, including its protein-protein interactions (PPIs) and post-translational modifications (PTMs), is crucial for developing therapeutic strategies. In this study, we employed high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) to systematically investigate the Mpro interactome and its modification status. To identify potential E3 ubiquitin ligases that regulate Mpro stability or function, we performed immunoprecipitation-mass spectrometry (IP-MS) using 293T cells expressing Flag-tagged Mpro. Additionally, we mapped the phosphorylation sites of Mpro to elucidate how host-cell kinases modulate its enzymatic activity. Our data revealed several key E3 ligases that interact with Mpro and identified novel phosphorylation sites on the protease. This dataset provides a comprehensive resource for understanding the host-virus interaction and the precise regulation of SARS-CoV-2 replication. |
| HostingRepository | iProX |
| AnnounceDate | 2026-03-02 |
| AnnouncementXML | Submission_2026-03-01_23:17:48.853.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yiwen Xie |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-01 23:17:10 | ID requested | |
| ⏵ 1 | 2026-03-01 23:17:49 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: IP-MS |
Contact List
| Lanjuan Li | |
|---|---|
| contact affiliation | The First Affiliated Hospital, Zhejiang University School of Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine |
| contact email | ljli@zju.edu.cn |
| lab head | |
| Yiwen Xie | |
| contact affiliation | The First Affiliated Hospital, Zhejiang University School of Medicine |
| contact email | xieeven@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
