⮝ Full datasets listing
PXD075065
PXD075065 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An orally active dual CBP/p300 degrader targets core dependencies of multiple myeloma |
| Description | Data were acquired using an in-house-developed method for global targeted proteomics of human proteins (UniProt UP000005640 extended with human UniParc proteins) implemented through the application programming interface (API) provided by Thermo Fisher Scientific. The method involved acquiring MS2 spectra upon peptide fragmentation with CID at 30 % normalized collision energy in the Orbitrap at a resolution of 50000 following collision-induced dissociation (CID). From each MS2 scan, up to ten of the most intense product ions were selected for subsequent MS3 analysis. Peptide-spectral matches were made in real-time using a custom algorithm and validated post-acquisition with a target-decoy search strategy to ensure a false discovery rate (FDR) of less than or equal to 1% (PMID: 17327847). Quantitative MS3 spectra were acquired in the Orbitrap at a resolution of 50,000. For quantification, TMT reporter ion intensities were extracted from the collected MS3 spectra by selecting the most intense signal within a 0.03 Th isolation window centered on the expected m/z for each reporter ion. A single global run was first performed followed by a targeted run. The cell line analyzed is MM1S and the drug used is PT69. Time points are laid out as below for each TMT Channel: 126,127n,127c : MMS1S_NoDrugControl_1Hour 128n,128c,129n : MMS1S_PT69_1Hour 129c,130n,130c : MMS1S_NoDrugControl_2Hour 131n,131c,132n : MMS1S_PT69_2Hour 132c,133n,133c : MMS1S_NoDrugControl_4Hour 134n,134c,135n : MMS1S_PT69_4Hour |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-07 |
| AnnouncementXML | Submission_2026-05-07_09:59:02.416.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Soroush Hajizadeh |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | TMTpro |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-01 14:35:40 | ID requested | |
| ⏵ 1 | 2026-05-07 09:59:03 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Orbitrap Eclipse, CBP, p300, Degradation, TMTPro, DatasetType:Proteomics |
Contact List
| Wilhelm Haas | |
|---|---|
| contact affiliation | Massachusetts General Hospital and Harvard Medical School |
| contact email | whaas@mgh.harvard.edu |
| lab head | |
| Christopher Ott | |
| contact affiliation | Massachusetts General Hospital and Harvard Medical School |
| contact email | Christopher.Ott@MGH.HARVARD.EDU |
| lab head | |
| Soroush Hajizadeh | |
| contact affiliation | MGH |
| contact email | roushrsh@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101009/ |
