⮝ Full datasets listing
PXD075033
PXD075033 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DNA pull down raw data of proteins pulled down by the biotinylated VE-Cadherin promoter under different lysis conditions |
| Description | The target promoter sequence (1000 bp upstream of exon) was retrieved from the NCBI database (https://www.ncbi.nlm.nih.gov/) and synthesized with 5' biotin modification (FITGENE, China). Tumor cells were lysed under two conditions: (1) SDS lysis buffer with sonication (strong condition) or (2) IP lysis buffer (mild condition). Lysates were centrifuged (14,000g, 10 min, 4 °C), then incubated overnight at 4 °C with biotinylated DNA, followed by streptavidin magnetic bead capture (30 min, RT). Bound proteins were eluted in 4× Laemmli buffer (95°C, 7 min), resolved on 10% Omni-PAGE™ Hepes Plus gels, and processed for LC-MS/MS proteomic analysis. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-28 |
| AnnouncementXML | Submission_2026-02-27_23:14:06.599.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiawen Cui |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-27 23:13:44 | ID requested | |
| ⏵ 1 | 2026-02-27 23:14:07 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: DNA pull down |
Contact List
| Jiali Liu | |
|---|---|
| contact affiliation | China Pharmaceutical University |
| contact email | 897813761@qq.com |
| lab head | |
| Jiawen Cui | |
| contact affiliation | China Pharmaceutical university |
| contact email | cuijw0514@outlook.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
