PXD075028 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Landscape and dynamics of TadA-dependent RNA editing in Escherichia coli reveal a role in nutrient-rich growth |
| Description | Adenosine-to-inosine A-to-I mRNA editing alters genetic information post-transcriptionally and can impact protein sequence and function, yet its regulation in bacteria remains unclear. Here, we profiled A-to-I editing in Escherichia coli across nutrient-rich LB and minimal M9 media and different growth phases. Our analysis expanded the repertoire of TadA-dependent A-to-I edited mRNAs to 27, including 12 novel sites, and revealed that editing levels were dynamic and markedly increased at stationary phase in LB but not in M9. Editing levels were independent of mRNA expression yet correlated with tRNA-Arg2 downregulation, and overexpressing tRNA-Arg2 reduced mRNA editing, demonstrating substrate competition for TadA, the sole bacterial tRNA adenosine deaminase. Mutants with TadA-deficient editing or reduced tRNA-Arg2 expression displayed similar LB-specific growth defects. Moreover, tRNA-Arg2 expression, tRNA-Arg2-dependent codon usage, and tRNA-Arg2 editing were all elevated in LB compared to M9. These findings establish regulatory principles for bacterial RNA editing, implicate tRNA editing in nutrient-responsive fitness, and provide a framework to explore the physiological roles of mRNA editing |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-24 |
| AnnouncementXML | Submission_2026-03-24_02:14:14.275.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dganit Melamed Kadosh |
| SpeciesList | scientific name: Escherichia coli (strain K12); NCBI TaxID: NEWT:83333; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-27 22:08:19 | ID requested | |
| ⏵ 1 | 2026-03-24 02:14:14 | announced | |
Publication List
Keyword List
| submitter keyword: TadA-dependent RNA editing, nutrient-rich growth |
Contact List
| Dan Bar-Yaacov |
|---|
| contact affiliation | 1The Shraga Segal Department of Microbiology, Immunology, and Genetics, Ben-Gurion University of the Negev, Israel |
| contact email | danbary@bgu.ac.il |
| lab head | |
| Dganit Melamed Kadosh |
|---|
| contact affiliation | Technion |
| contact email | sdganit@tx.technion.ac.il |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075028
- Label: PRIDE project
- Name: Landscape and dynamics of TadA-dependent RNA editing in Escherichia coli reveal a role in nutrient-rich growth
