PXD074993

PXD074993 is an original dataset announced via ProteomeXchange.

Title	Htr2b Enhances the Phagocytosis of Brain Macrophages and Alleviates Brain Injury after Stroke
Description	Brain myeloid cells, including infiltrating monocyte-derived macrophages (MDMs) and resident microglia play critical roles in regulating debris clearance following ischemic brain injury, shaping neuroinflammation evolution and neurological outcome, yet the underlying mechanism remains elusive. In this study, using single-cell RNA sequencing and fate mapping, we identified serotonin receptor Htr2b+ MDMs as a pivotal subgroup involved in resolving neuroinflammation within the ischemic penumbra. In addition, we find significant accumulation of Htr2b+ MDMs with enhanced phagocytosis and inflammation-resolving gene signatures in the peri-infarct region. Mechanistically, Htr2b activation promotes nuclear translocation of Tfe3, a key lysosomal regulator, enhancing lysosomal biogenesis and supporting sustained phagocytic activity. Myeloid-specific Htr2b ablation impairs debris clearance, exacerbates neuroinflammation and ischemic injury, while pharmacological Htr2b agonism or Tfe3 activation restores the lysosomal competence of brain MDMs and improves post-stroke neurological outcome. In conclusion, we demonstrate a novel Htr2b+ MDM subset that integrates serotonin signaling to license their intrinsic phagocytosis function, alleviating ischemic brain injury.
HostingRepository	PRIDE
AnnounceDate	2026-07-04
AnnouncementXML	Submission_2026-07-04_07:35:17.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yonghui chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-27 05:14:11	ID requested
⏵ 1	2026-07-04 07:35:18	announced

Dataset with its publication pending

submitter keyword: Ischemic stroke

monocyte-derived macrophages

Htr2b

Tfe3

phagocytosis

Yueman zhang
contact affiliation	Department of Anesthesiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
contact email	altas_zhang@163.com
lab head
yonghui chen
contact affiliation	Renji Hospital, Shanghai Jiao Tong University School of Medicine‌
contact email	cyhgordon@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD074993

PRIDE project URI

• PRIDE
  1. PXD074993
     1. Label: PRIDE project
     2. Name: Htr2b Enhances the Phagocytosis of Brain Macrophages and Alleviates Brain Injury after Stroke