PXD074990

PXD074990 is an original dataset announced via ProteomeXchange.

Title	Ubiquitin substrates were identified in SILAC-labeled H1299 wild-type and MPND KO cells using LC-MS/MS.
Description	Using H1299 wild-type cells and their MPND-knockout derivatives as research subjects, this project employs Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for metabolic labeling of the two cell groups. Combined with ubiquitinated peptide-specific enrichment and nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques, we aim to establish a quantitative expression profile of ubiquitin proteins in these cells. Subsequent data retrieval, quantitative analysis, and differential screening will be performed using software such as MaxQuant to clarify the differential expression characteristics of ubiquitin proteins in H1299 cells following MPND knockout, including up-regulated and down-regulated ubiquitin proteins as well as specific ubiquitination modification sites. Furthermore, bioinformatics analyses—including Gene Ontology (GO) functional enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and protein-protein interaction (PPI) network construction—will be utilized to predict the signaling pathways involved in these differential ubiquitin proteins and the potential downstream substrates regulated by MPND.
HostingRepository	PRIDE
AnnounceDate	2026-05-15
AnnouncementXML	Submission_2026-05-14_22:31:32.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dawei Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-27 03:13:19	ID requested
⏵ 1	2026-05-14 22:31:33	announced

Dataset with its publication pending

submitter keyword: H1299,MPND

Dawei Li
contact affiliation	Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou Medical College of Soochow University
contact email	daweili@suda.edu.cn
lab head
Dawei Li
contact affiliation	The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou Medical College of Soochow University
contact email	daweili@suda.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074990
     1. Label: PRIDE project
     2. Name: Ubiquitin substrates were identified in SILAC-labeled H1299 wild-type and MPND KO cells using LC-MS/MS.